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dc.contributor.advisorBelshan, Michael A.en_US
dc.contributor.authorSanford, Bridget Lanelleen_US
dc.date.accessioned2012-12-19T21:00:51Z
dc.date.available2014-12-12T09:40:09Z
dc.date.issued2012-12-12en_US
dc.identifier.otherSanford_Bridget_Thesis.pdf
dc.identifier.urihttp://hdl.handle.net/10504/35663
dc.description.abstractThe human immunodeficiency virus type 1 (HIV-1) Matrix (MA) protein, a component of the major structural protein Gag, has been shown to play a role in both the early stages and late stages of the viral life cycle. Functional domains within the MA protein have been identified through numerous mutagenesis studies. In these studies, I investigated the effects of deletions in the C-terminal alpha helix of MA. Removal of amino acids 96 to 120 (MAΔ96-120) resulted in a loss of particle release. Moreover, MAΔ96-120 trans-dominantly inhibited wild type particle release in co-transfection assays. MAΔ96-120 was found to target properly to membranes in membrane floatation assays and to multimerize with wild-type Gag. Electron micrographs revealed the loss of release was due to particle retention at the plasma membrane. Subtilisin treatment failed to release virus, indicating the phenotype was not due to a protaceous factor. The infectivity of the mutants was measured with HIV or vesicular stomatitis virus VSV envelope proteins. All but one (MAΔ107-120) deletion mutant were non-infectious with either envelope. Taken together, these results demonstrate that the C-terminal alpha helix of MA, specifically amino acids 96 to 120, is critical for both virus release and the early steps of HIV infection.en_US
dc.language.isoen_USen_US
dc.publisherCreighton Universityen_US
dc.rightsCopyright is retained by the Author. A non-exclusive distribution right is granted to Creighton University and to ProQuest following the publishing model selected above.en_US
dc.subject.meshAnti-HIV Agents--pharmacologyen_US
dc.subject.meshHIV-1--drug effectsen_US
dc.titleThe C-Terminus of HIV-1 Matrix Contains a Domain Critical for Virus Releaseen_US
dc.typeThesis
dc.rights.holderBridget Sanforden_US
dc.publisher.locationOmaha, Nebraskaen_US
dc.description.noteProQuest Traditional Publishing Optionen_US
dc.description.pagesix, 58 pagesen_US
dc.contributor.cuauthorSanford, Bridget Lanelleen_US
dc.embargo.terms2014-12-12
dc.degree.levelMS (Master of Science)en_US
dc.degree.disciplineMedical Microbiology and Immunology (graduate program)en_US
dc.degree.nameM.S. in Medical Microbiology and Immunologyen_US
dc.degree.grantorGraduate Schoolen_US
dc.degree.committeeBartz, Jason C.en_US


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